PrinCE 250 and PrinCE-C 255: The basic model

The PrinCE 250 and the PrinCE-C 255 are the basic capillary electrophoresis systems. Both systems are fully automated and can handle up to three samples without interference of the operator, while still maintaining 1 buffer vial. As long as sample capacity is not a very important factor, this system is perfectly suitable for routine analysis.

The extra features of PrinCE-C 255, compared to the PrinCE 250:

• The effective capillary length is reduced by 5 cm.
• High pressure up to 10 bars by gas pressure allows CEC and faster rinsing with buffers of high viscosity (or: use of buffers with even higher viscosity, use of high viscosity buffers in narrower i.d. capillaries).

Main Specifications:

-  Flushing pressure	0 to +3000 mbar by DCI (PrinCE 250) 0 to +2500 mbar by DCI device, up to 10 bar by external pressure (PrinCE-C 255)
-  Voltage range	-30 kV to +30 kV
-  Current range	-200 μA to + 200 μA
-  Ramping	Programmable voltage or current ramping
-  Polarity reversal	Programmable pre-run and during the run
-  Autosampler	4 position sample and buffer vials. Variable vial sizes for samples and buffers available, including Eppendorf micro centrifuge tubes
-  Temperature control	Capillary 5°C below ambient to 60°C
-  Detector	Allows the use of any CE-detector including MS, Fluorescence, Conductivity, UV/VIS, and Photo Diode Array
-  Software	Allows the use of any data station. Optional: Our DAx Data Acquisition and Analysis software. This software is specially made for our PrinCE systems

Injection modes Hydrodynamic injection uses patended Dynamic Compression Injection (DCI) to generate and apply a range of positive and negative pressures with controlled ramping (-180 to 250 mbar, 1 mbar resolution). Electrokinetic Injection features controlled ramping in voltage or current mode (max. 30 kV).